Search results for "Cell culture supernatant"
showing 3 items of 3 documents
Quantitative studies of the secretion of complement component C3 by resident, elicited and activated macrophages. Comparison with C2, C4 and lysosoma…
1982
To quantitate the secretion of complement component C3 by guinea pig peritoneal macrophages an enzyme-linked immunosorbent assay was developed. C3 secretion was studied in resident, elicited and activated macrophages and compared with release of hemolytically active C2 and C4, as well as the lysosomal enzyme β-D-2-acetamido-2-deoxyglucosidase. Resident macrophages secreted about 6 ng C3/106 cells/h into culture supernatants over a period of 12 h. Corynebacterium parvum-activated cells were found to secrete 3 times that amount at nearly constant rates. There was a stepwise increase in secretion of functional C2 and C4 when comparing resident, elicited and activated macrophages; secretion was…
Granulocyte Activity Is Enhanced by Culture Supernatants of Mononuclear Leukocytes Incubated with Tetrachlorodecaoxide
1995
Granulocytes (polymorphonuclear leukocytes, PMNs) and monocytes/ macrophages are important in wound healing. Within the first few hours PMNs and monocytes begin to infiltrate sites of injury. The major function of PMNs is to kill microbes and phagocytose cellular debris so that healing can proceed [1]. Monocytes/macrophages invading the wound are also active in phagocytosis. In addition they release a number of cytokines including growth factors, interleukin-8 (IL-8), and tumor necrosis factor (TNF-α) needed for new tissue formation and shown to stimulate PMNs [1–3].
Leukocyte chemotactic activity in cultures of unstimulated human lymphocytes.
2009
We have shown earlier that unstimulated human lymphocytes in in vitro cultures produce migration inhibitory factor into the supernatant. The evidence of spontaneous lymphokine synthesis is strengthened further by this study, which demonstrates leukocyte chemotactic activity in these culture supernatants. The factor has a molecular weight of more than 5000 daltons, it resisted heating for 15 min at 100 degrees C, and showed maximum activity at dilution 1:4-1:8 of the supernatants.